MORPHOLOGICAL CHARACTERIZATION OF Trichoderma spp. AND THEIR EFFICACY AS BIOCONTROL AGENTS AGAINST Ganoderma boninense In Vitro

Abstract

Trichoderma species act as biological control agents against fungal plant pathogens either directly or directly. In this study, dual culture was used to assess the potential of Trichoderma asperellum, T. harzianum, T. brevicompatum and T. viren to control Ganoderma boninense. These species were morphologically and Potato dextrose agar (PDA) and maltextract agar (MEA) supported the highest mycelia growth. provides useful information on the sporulation. Antagonistic effects of Trichoderma spp. against G. boninense mycelia growth were evaluated using dual culture test. The results demonstrated that the percentage inhibition of radial growth(PIRG) of G. boninense was >75% in in-vitro assay. The highest percentage inhibition of radial (PIRG) values was observed in T. asperellum (84.1%), while the minimum colony over growth was observed in T. brevicompatum (76%). This study showed that Trichoderma spp. have a good antagonistic effect on G. boninense mycelia growth and T. asperellum has the best potential to control the basal stem rot disease pathogen.